Microarray analysis of eyes only from 23-hpf wild-type and pitx2M64* homozygous mutant zebrafish embryos

ABSTRACT: We have developed a pitx2 knockout zebrafish line (c.190_197delATGTCGAC, p.(Met64*)) that recapitulates the characteristic phenotypes of PITX2-mediated Axenfeld-Rieger syndrome (ARS) as well as displays some unique ocular phenotypes that in humans have implicated possible PITX2 involvement.To begin to ascertain the downstream pathways that are disrupted in pitx2M64* homozygous mutants, RNA from 23-hpf wild-type and pitx2M64* homozygous eyes only was submitted for microarray analysis.To collect embryonic ocular tissues from mutant and wild-type embryos, whole eyes of 23-hpf embryos were dissected; wild-type and pitx2M64* homozygous mutant whole eyes were then pooled together respectively for RNA extractions. RNA was extracted from the collected eyes using TRIzol, assessed for quality and as

ORGANISM(S): Danio rerio

SUBMITTER: Elena Semina 

PROVIDER: E-MTAB-6463 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress