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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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ChIP-seq of IDS1-myc using Oryza sativa seedlings

ABSTRACT: IDS1 is a rice AP2-type transcription factor with transcritpional repression activity. To understand how IDS1 regulate rice salt tolerance, the ChIP-seq experiments were performed to identify IDS1 binding site in globle genomic level. The two-weeks-old rice seedlings were lysated and sonificated and IDS1-DNA complexes were immune precipated with myc-antibody and protein A beads. The purified DNA samples were used to construct sequencing libraries and sequenced with Illumina. The data were then analyzed with bio-informatic tools.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Oryza sativa Japonica Group

SUBMITTER: Xiliu Cheng

PROVIDER: E-MTAB-6504 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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