ChIP-Seq of fly Schneider S2 cells to investigate CID islands

ABSTRACT: The centromere-specific Histone H3-variant CENH3 (also known as CENP-A) is considered to be an epigenetic mark for establishment and propagation of centromere identity. Pulse-induction of CENH3 (Drosophila CID) in Schneider S2 cells incorporates into noncentromeric regions and generates CID islands that resist clearing from chromosome arms for multiple cell generations. We demonstrate that CID islands represent functional ectopic kinetochores, which are non-randomly distributed on the chromosome and display a preferential localization near telomeres and pericentric heterochromatin in transcriptionally silent, intergenic chromatin domains. Although overexpression of heterochromatin protein 1 (HP1) or increasing Histone acetylation interferes with CID islands formation on a global scale, ind

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Sarah Diehl 

PROVIDER: E-MTAB-652 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress