MiRNA profiling of sarcoidosis Treg cells

ABSTRACT: Sarcoidosis is characterized by exaggerated immune response to unknown agent and can affect different organs. One of the main players in the pathology of the disease are regulatory T cells (Tregs), however up to date there are no insides on the possible molecular alterations of this particular cell subset. Therefore, in the current study we looked for the global changes on miRNA and mRNA level of Tregs of patients with the most predominant form of the disease - pulmonary sarcoidosis (PS). For this purpose we sorted Treg cells from peripheral blood (PB) and bronchoalveolar lavage (BAL) and analyzed the global molecular changes. MiRNA analysis showed major changes in Tregs isolated from PB to the ones from BAL, pointing at the stronger impact of the microenvironment than the disease state. A

INSTRUMENT(S): Qubit, OpenArray NT cycler, PCR Applied Biosystems

ORGANISM(S): Homo sapiens

SUBMITTER: Neli Kachamakova-Trojanowska 

PROVIDER: E-MTAB-6590 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress