Transcription profiling of Corynebacterium glutamicum shows a differential response to moderate or severe heat shock of the HrcA- and HspR-dependent systems

ABSTRACT: Genome-wide transcription profiles analysis of the heat-shocked wild-type strain under moderate (40¡C) and severe heat stress (50¡C) revealed that a large number of genes are differentially expressed after heat shock. 358 genes were up-regulated and 420 were down-regulated in response to moderate heat shock 35 (40¡C) in C. glutamicum. Our results confirmed the HrcA/CIRCE-dependent and HspR/HAIR-dependent up-regulation of chaperones following heat shock. Other genes including COG groups related to macromolecule biosynthesis and several transcriptional regulators (COG class K) were up-regulated, explaining the large number of genes affected by heat shock. Mutants having deletions in the hrcA or hspR regulators were constructed, which allowed the complete identification of the genes controlled by those systems. The up- or down-regulation of several genes observed in the microarray experiments was validated by Northern analyses and quantitative (real-time) PCR. These analyses showed the heat shock intensity-dependent response (differential response) presented by the HspR/HAIR system, in contrast with the non-differential response shown by the HrcA/CIRCE-regulated genes.

ORGANISM(S): Corynebacterium glutamicum

SUBMITTER: Jšrn Kalinowski 

PROVIDER: E-MTAB-66 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress