Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Analysis of the impact of extracellular acidosis on the expression of miRNAs in experimental tumor lines LLC-WRC 256 and R-3327-AT-1 of the rat

ABSTRACT: Hypoxia has been shown to regulate the miRNA expression in tumors. Since many tumors show a extracellular acidosis the aim of the study was to analyze whether the extracellular pH has an impact on the miRNA expression in experimental tumor cells of the rat. Therefore R3327-AT1 Dunning prostate carcinoma and Walker-256 mammary carcinoma cells were incubated at pH 7.4 and 6.6 for 24 and the miRNA expression was analyzed by NGS.

INSTRUMENT(S): Illumina HiScanSQ

ORGANISM(S): Rattus norvegicus

SUBMITTER: Oliver Thews

PROVIDER: E-MTAB-6600 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Acidic extracellular environment affects miRNA expression in tumors in vitro and in vivo.

Riemann Anne A Reime Sarah S Thews Oliver O

International journal of cancer 20181218 7

Hypoxia can control the expression of miRNAs in tumors which play an important role for the control of the malignant behavior. The aim of the study was to analyze whether extracellular acidosis, a common feature of tumors, also has an impact on the miRNA expression in isolated cells as well as in solid tumors. MiRNA expression was analyzed in two rat tumor cell lines (AT1 prostate and Walker-256 mammary carcinomas) by NGS and qPCR. In vivo the same cell lines were implanted subcutaneously and th ...[more]

PMID: 30098207

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Dataset Information

Analysis of the impact of extracellular acidosis on the expression of miRNAs in experimental tumor lines LLC-WRC 256 and R-3327-AT-1 of the rat

Publications

Acidic extracellular environment affects miRNA expression in tumors in vitro and in vivo.

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