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Unknown,Transcriptomics,Genomics,Proteomics

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A simple and robust plate-based single-cell ATAC-seq on various tissues and cell lines

ABSTRACT: The assay for transposase-accessible chromatin using sequencing (ATAC-seq) is widely used to identify regulatory regions throughout the genome. However, only a few studies have been done at the single cell level (scATAC-seq) due to technical difficulties. Here we developed a simple and robust plate-based scATAC-seq method, combining upfront bulk tagmentation with single-nuclei sorting, to investigate open chromatin regions. We applied this method on mouse splenocytes and unbiasedly revealed key regulatory regions and transcription factors that define each cell (sub)type.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): mixed sample

SUBMITTER: Xi Chen

PROVIDER: E-MTAB-6714 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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