Microarray of isolated adipocytes from subcutaneous adipose tissue of severely obese patients compared to lean controls

ABSTRACT: The aim of the project was to compare global gene expression in adipocytes from obese patients and lean controls. Subcutaneous adipose tissue was collected from severely obese patients undergoing bariatric surgery (average body-mass index (BMI) of 45.5 kg/m2 (n = 12, thereof 4 men) and healthy lean patients undergoing hernia repairs (average BMI of 24.2 kg/m2 (n = 12, thereof 7 men), between 27 and 56 years of age. Adipocytes were isolated by collagenase treatment of adipose tissue, followed by filtering and centrifugation. Floating adipocytes were lysed in Qiazol before RNA purification and microarray analysis.

ORGANISM(S): Homo sapiens

SUBMITTER: Simon Dankel 

PROVIDER: E-MTAB-6728 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress