Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Development of Resistant Tumors to Anti-angiogenic Therapy (SU 11248) via in-vivo RNAi Screen

ABSTRACT: We sought to decipher the molecular landscape of tumor resistance to continuous treatment with VEGF-receptor tyrosine kinase inhibitor (RTKi) sunitinib (SU11248, SU). To address that, we performed serial transplantation of a syngeneic LLC tumor models in C57bl6 mice using a genome-wide lentiviral shRNA library targeting about 39,000 transcripts (150K complexity). The abundance of each single knock down (kd) was longitudinally traced via molecular barcodes towards development of resistant tumors. After three in-vivo panning rounds, distinct shRNA constructs were found to be significantly enriched in tumors passaged under SU selection pressure (p3-SU) compared to those passaged without treatment (p3-ctrl).

ORGANISM(S): Mus musculus

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PROVIDER: E-MTAB-6749 | biostudies-arrayexpress |

SECONDARY ACCESSION(S): E-MTAB-6745

REPOSITORIES: biostudies-arrayexpress

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Development of Resistant Tumors to Anti-angiogenic Therapy (SU 11248) via in-vivo RNAi Screen

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