Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

8 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Timecourse analysis of fission yeast CSL mutants treated with hydrogen peroxide

ABSTRACT: Fission yeast CSL mutants (cbf11 and cbf12 knock-outs) show altered sensitivity to hydrogen peroxide. To help elucidate the molecular basis of this phenomenon, we analyzed wild-type and mutant transcriptomes before stress and as a timecourse after treatment with hydrogen peroxide. Cells were grown to exponential phase in YES medium, untreated samples were collected (time 0), cells were treated with 0.74 mM hydrogen peroxide and harvested after 15 and 60 min post treatment. Total RNA was isolated, labelled and hybridized to custom-made two-colour microarrays. Each sample was hybridized against reference created by pooling equal aliquots from all 9 samples.

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Martin Převorovský

PROVIDER: E-MTAB-6761 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Transcriptome analysis (Agilent microarrays) of WT, cbf11 and cbf12 Schizosaccharomyces pombe strains treated with camptothecin

Project description:The aim was to determine expression levels in untreated cells and cells treated with 20 micromolar camptothecin (CPT) for 2 hours. And the to compare the cbf11 and cbf12 deletion mutants with WT. Cells were grown to exponential phase in YES at 32°C, aliquots of cultures were collected (sample \\"control\\"), remaining cells were treated with CPT (dissolved in DMSO) and harvested 2 hours later. Two independent biological experiments were performed. A pooled RNA sample was prepared for each independent run that contained aliquots of all 6 individual RNA samples. RNA was hybridized on 2-colour Agilent microarrays against the corresponding pooled sample (with dye swap).

2024-04-05 | E-MTAB-13621 | biostudies-arrayexpress

Cbf11 Regulates Periodic Expression of Genes Required to Prevent Catastrophic Mitosis in the Fission Yeast Cell Cycle

Project description:The expression of a large proportion of the fission yeast genome changes periodically with the cell cycle. Several key transcription factors have been identified that regulate these oscillating and interdependent waves of gene expression. However, for a significant number of cell cycle-regulated genes the regulator(s) driving these oscillations remain unknown. Cbf11 and Cbf12, the fission yeast CSL transcription factors, have been implicated in the regulation of cell cycle progression, yet the details of their functioning are poorly understood. Using a combination of transcriptome profiling and genome-wide mapping of CSL-DNA interactions we have identified a comprehensive set of CSL-regulated genes. Our data indicate that Cbf11 and Cbf12 contribute directly and indirectly to the regulation of periodically expressed genes in fission yeast. We show that during S phase/cytokinesis Cbf11 directly activates the transcription of several periodic genes required in the cell to prevent catastrophic mitosis. In agreement with these findings, multiple aspects of cell cycle progression are perturbed when CSL cellular levels are genetically manipulated. We have identified Cbf11 as a novel cell cycle phase-specific activator of genes required for proper coordination of cell and nuclear division, and prevention of catastrophic mitosis in fission yeast.

2015-09-18 | E-MTAB-2724 | biostudies-arrayexpress

Transcription profiling of mouse macrophage responses to all-trans retinoic acid between wild type and NCoR-/- mice

Project description:Comparison of mouse macrophage responses to all-trans retinoic acid between wild-type and NCoR-/- mice.

2006-11-06 | E-MEXP-37 | biostudies-arrayexpress

Transcription profiling of mouse macrophage responses to 12-o-tetradecanoylphorbol-13-acetate (TPA), lipopolysaccharide (LPS), and LPS plus dexamethasone between wild type and NCoR-/- mice

Project description:Comparison of mouse macrophage responses to 12-o-tetradecanoylphorbol-13-acetate (TPA), lipopolysaccharide (LPS), and LPS plus dexamethasone between wild-type and NCoR-/- mice.

2004-08-01 | E-MEXP-39 | biostudies-arrayexpress

Transcription profiling of mouse macrophage responses to lipopolysaccharide (LPS) and 12-o-tetradecanoylphorbol-13-acetate (TPA) in wild type and NcoR-/- mice

Project description:Comparison of mouse macrophage responses to lipopolysaccharide (LPS) and 12-o-tetradecanoylphorbol-13-acetate (TPA) between wild-type and NcoR-/- mice.

2006-11-06 | E-MEXP-38 | biostudies-arrayexpress

Transcription profiling of rat mixed-tissue RNA samples using different labeling protocols

Project description:At one site (#10), three different batches of MTRRM (see E-TABM-16), were labeled with two different kits (Enzo and Affymetrix) and hybridized to two different Affymetrix Arrays (RAE230A and RAE230_2).

2008-04-16 | E-MEXP-1563 | biostudies-arrayexpress

Transcription profiling of rat mixed-tissue RNA samples over 8.5 months to monitor processing drift

Project description:Process drift at one site was monitored by assaying the rat Mixed Tissue RNA Reference Material (see E-TABM-16) multiple times a week for 8.5 months.

2008-04-16 | E-MEXP-1565 | biostudies-arrayexpress

Transcription profiling of rat heart from a spontaneously hypertensive strain treated with doxorubicin or dexrazoxane and then doxorubicin

Project description:This study was initiated to characterize early DOX-induced changes in cardiac gene expression in order to identify potential additional areas for clinical intervention. Male spontaneously hypertensive rats (SHR) received 3 mg/kg DOX or vehicle (iv) 30 minutes following pretreatment with 50 mg/kg DZR or saline (ip) weekly for 1, 2 or 3 weeks.

2009-06-01 | E-MEXP-2071 | biostudies-arrayexpress

Transcription profiling of fresh and frozen rat liver RNA samples to explore how RNA quality affects microarray results

Project description:To systemically explore how RNA quality affects microarray assay results, a set of rat liver RNA samples with a progressive change in RNA quality was generated either by thawing frozen tissue or by ex vivo incubation of fresh tissue.

2007-09-01 | E-MEXP-1069 | biostudies-arrayexpress

miRNA array of 5 plasma with recurrent stage II gastric cancer against 5 plasma non-recurrent stage III gastric cancer in humans

Project description:1ml plasma from 10 age- and sex-matched patients (GC stage II with recurrence: sample number 1-5; and stage III without recurrence: sample number 6-10) were arrayed for differential expressed miRNAs so as to look for miRNAs that were associated with GC recurrence. Raw CT values were shown in this dataset.

2017-08-18 | E-MTAB-5854 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data