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Changes in gene expression when the redox potential of steady state cultures of Staphylococcus aureus is lowered by addition of dithiothreitol

ABSTRACT: Staphylococcus aureus can survive in the low redox potential environment of the host cytosol. How low redox potential affects S. aureus USA300 Je2 was investigated by establishing aerobic steady state chemostat cultures and then perturbing the steady state by addition of the reducing agent dithiothreitol. These highly controlled steady state cultures allowed only one parameter (redox potential) changed. Dithiothreitol was used as a non-metabolizable reductant. Before and after perturbation samples were taken for gene expression profiling. Three independent biological replicates were analyzed.

ORGANISM(S): Staphylococcus aureus subsp. aureus USA300

SUBMITTER: Jeffrey Green

PROVIDER: E-MTAB-6924 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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