Single cell RNA-sequencing of primary mouse Neural Plate Border Stem Cells

ABSTRACT: We describe a so far uncharacterized, embryonic and self-renewing Neural Plate Border Stem Cell (NBSC) population with the capacity to differentiate into central nervous and neural crest lineages. NBSCs can be obtained by neural transcription factor-mediated reprogramming (BRN2, SOX2, KLF4, and ZIC3) of human adult dermal fibroblasts and peripheral blood cells (induced Neural Plate Border Stem Cells, iNBSCs) or by directed differentiation from human induced pluripotent stem cells (NBSCs). Moreover, human (i)NBSCs share molecular and functional features with an endogenous NBSC population isolated from neural folds of E8.5 mouse embryos. Upon differentiation, iNBSCs give rise to either (1) radial glia-type stem cells, dopaminergic and serotonergic neurons, motoneurons, astrocytes, and oligod

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Mus Musculus

SUBMITTER: Andreas Trumpp 

PROVIDER: E-MTAB-6925 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress