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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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SCRB-seq comparison with TruSeq on bulk RNA-seq human LCL (DMSO) and BAY treated LCL (BAY)

ABSTRACT: Here, we validate a novel protocol, Bulk RNA Barcoding and sequencing (BRB-seq), that combines the multiplexing-driven cost-effectiveness of a single-cell RNA-seq protocol with the efficiency of a bulk RNA-seq procedure. For this we first gauge the applicability of the SCRB-seq protocol on bulk DMSO and BAY treated human LCL, and compare its effectiveness as compared to TruSeq.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Homo sapiens

SUBMITTER: Vincent Gardeux

PROVIDER: E-MTAB-6984 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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BRB-seq: ultra-affordable high-throughput transcriptomics enabled by bulk RNA barcoding and sequencing.

Alpern Daniel D Gardeux Vincent V Russeil Julie J Mangeat Bastien B Meireles-Filho Antonio C A ACA Breysse Romane R Hacker David D Deplancke Bart B

Genome biology 20190419 1

Despite its widespread use, RNA-seq is still too laborious and expensive to replace RT-qPCR as the default gene expression analysis method. We present a novel approach, BRB-seq, which uses early multiplexing to produce 3' cDNA libraries for dozens of samples, requiring just 2 hours of hands-on time. BRB-seq has a comparable performance to the standard TruSeq approach while showing greater tolerance for lower RNA quality and being up to 25 times cheaper. We anticipate that BRB-seq will transform ...[more]

PMID: 30999927

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