ScRNA-seq to investigate endoderm differentiation from induced human pluripotent stem cells

ABSTRACT: Single-cell RNA-seq (scRNA-seq) on nocodazole and DMSO treated cells before and after differentiation into endoderm. hPSC colonies were treated with DMSO or 100ng/ml nocodazole for 16 hours and induced to differentiate into definitive endoderm for three days. Single cells were subsequently collected in either undifferentiated conditions (Und) or after 3 days of endoderm differentiation (Endoderm) and then sorted onto 384 well plates for Smart-Seq2 processing.

INSTRUMENT(S): Illumina HiSeq 4000

ORGANISM(S): Homo sapiens

SUBMITTER: Daniele Muraro 

PROVIDER: E-MTAB-7008 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress