RNA-Seq of human fetal hearts at 9, 12 and 16 weeks of gestation

ABSTRACT: RNA-Seq was used to study alterations in gene expression in the developing human heart across three gestational ages in the first and early second trimester. All tissue was acquired following elective termination of pregnancy and informed parental consent. Storage and use of tissue was in premises licensed by the 2004 Human Tissues Act (UK) and all protocols had ethical committee approval. All samples were found to be morphologically normal during regular checks with the midwife and upon medical termination of pregnancy as certified by the physician. RNA was extracted using BioRad Aurum Total RNA Fatty and Fibrous Tissue Kit (cat no. 732-6830) following manufacturers instructions. Illumina compatible barcoded mRNA sequencing libraries were made from 100 ng of total RNA after polyA selectio

INSTRUMENT(S): Illumina HiSeq 3000

ORGANISM(S): Homo sapiens

SUBMITTER: Dr Ian Carr 

PROVIDER: E-MTAB-7031 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress