Transcription start site profiling of a Streptococcus pyogenes emm1 strain

ABSTRACT: The human pathogen Streptococcus pyogenes, or group A streptococcus, is responsible for mild infections to life-threatening diseases. To determine the primary transcriptome of the emm1 strain S119, we have performed a differential RNA-Seq experiment based on selective Tobacco Acid Pyrophosphatase (TAP) treatment and 5' adapter ligation to differentiate primary transcripts (5' tri-phosphate) and processed RNAs (5' mono-phosphate). The libraries were performed on a mixture of RNAs prepared from bacteria cultured to late exponential phase in a rich growth culture medium supplemented or not with 15 mM of MgCl2

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Streptococcus pyogenes

SUBMITTER: Rosinski-Chupin Isabelle 

PROVIDER: E-MTAB-7099 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress