MiRNA microarray of human pulmonary endothelial cells comparing patients with and without Chronic Obstructive Pulmonary Disease.

ABSTRACT: This experiment was carried out to see if there were any miRNA expression differences in pulmonary endothelial cells between patients with and without COPD. COPD is an inflammatory condition and although much work has previously been performed to investigate the inflammatory cells in COPD there has not been as much research looking at the endothelium through which inflammatory cells must pass through to reach the lung tissue. In this experiment pulmonary endothelial cells were extracted from whole lung tissue removed at the time of cardiothoracic surgery. This was performed for patients with and without COPD. RNA was extracted using the Qiagen microRNeasy kits prior to transferring to the University of Birmingham Biosciences department who performed RNA labelling and ran the microarrays. O

ORGANISM(S): Homo sapiens

SUBMITTER: Clara Green 

PROVIDER: E-MTAB-7141 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress