FloChIP's decreasing cell number data obtained in multiplexing mode
Ontology highlight
ABSTRACT: By using FloChIP’s “sample multiplex” mode, we ChIPed in parallel 4 different cell dilution (100k cells, 50k cells, 5k cells, and 500 cells), going from chromatin to sequencing-ready libraries, in just one day.
Project description:By using FloChIP’s “sample multiplex” mode, we ChIPed in parallel 5 histone marks (H3K27ac, H3K4me3, H3K4me1 and H3K9me3), going from chromatin to sequencing-ready libraries, in just one day.
Project description:By using FloChIP’s “sample multiplex” mode, we ChIPed in parallel 5 histone marks (H3K27ac, H3K4me3, H3K27me3, H3K4me1 and H3K9me3), going from chromatin to sequencing-ready libraries, in just one day.
Project description:By using FloChIP’s in sequential chip mode, we ChIPped multiple histone marks and re-ChIPped h3k27me3 and h3k4me3, in just one day.
Project description:Dressing electronic quantum states with virtual photons creates exotic effects ranging from vacuum-field modified transport to polaritonic chemistry, and squeezing or entanglement of modes. The established paradigm of cavity quantum electrodynamics maximizes the light-matter coupling strength ΩR/ωc , defined as the ratio of the vacuum Rabi frequency and the frequency of light, by resonant interactions. Yet, the finite oscillator strength of a single electronic excitation sets a natural limit to ΩR/ωc . Here, we enter a regime of record-strong light-matter interaction which exploits the cooperative dipole moments of multiple, highly non-resonant magnetoplasmon modes tailored by our metasurface. This creates an ultrabroadband spectrum of 20 polaritons spanning 6 optical octaves, calculated vacuum ground state populations exceeding 1 virtual excitation quantum, and coupling strengths equivalent to ΩR/ωc=3.19 . The extreme interaction drives strongly subcycle energy exchange between multiple bosonic vacuum modes akin to high-order nonlinearities, and entangles previously orthogonal electronic excitations solely via vacuum fluctuations.
Project description:Optical fiber communications rely on multiplexing techniques that encode information onto various degrees of freedom of light to increase the transmission capacity of a fiber. However, the rising demand for larger data capacity is driving the need for a multiplexer for the spatial dimension of light. We introduce a mode-division multiplexer and demultiplexer design based on a metasurface cavity. This device performs, on a single surface, mode conversion and coupling to fibers without any additional optics. Converted modes have high fidelity due to the repeated interaction of light with the metasurface's phase profile that was optimized using an inverse design technique known as adjoint analysis. We experimentally demonstrate a compact and highly integrated metasurface-based mode multiplexer that takes three single-mode fiber inputs and converts them into the first three linearly polarized spatial modes of a few-mode fiber with fidelities of up to 72% in the C-band (1530-1565 nm).
Project description:Metasurfaces are promising two-dimensional metamaterials that are engineered to provide unique properties or functionalities absent in naturally occurring homogeneous surfaces. Here, we report a type of metasurface for tailored reconstruction of surface plasmon waves from light. The design is based on an array of slit antennas arranged in a way that it matches the complex field distribution of the desired surface plasmon wave. The approach is generic so that one can readily create more intricate designs that selectively generate different surface plasmon waves through simple variation of the wavelength or the polarization state of incident light. The ultra-thin metasurface demonstrated in this paper provides a versatile interface between the conventional free-space optics and a two-dimensional platform such as surface plasmonics.