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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Single-cell transcriptional analysis reveals ILC-like cells in zebrafish

ABSTRACT: Transcriptome data from zebrafish single cells from guts from either from Tg(lck:EGFP) rag1-/-mutant or wild-type zebrafish were isolated and single cell suspensions were prepared as described in protocol section. Three zebrafish, per each condition (i.e. zebrafish intraperitoneally injected with PBS, lyophilised Anisakis simplex or inactivated Vibrio anguillarum), were used to collect the total of 12,000 lck+ cells (4000 per zebrafish) for 10x experiment.

INSTRUMENT(S): Illumina HiSeq 4000

ORGANISM(S): Danio rerio

SUBMITTER: Ana Cvejic

PROVIDER: E-MTAB-7159 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Single-cell transcriptional analysis reveals ILC-like cells in zebrafish.

Hernández Pedro P PP Strzelecka Paulina M PM Athanasiadis Emmanouil I EI Hall Dominic D Robalo Ana F AF Collins Catherine M CM Boudinot Pierre P Levraud Jean-Pierre JP Cvejic Ana A

Science immunology 20181101 29

Innate lymphoid cells (ILCs) are important mediators of the immune response and homeostasis in barrier tissues of mammals. However, the existence and function of ILCs in other vertebrates are poorly understood. Here, we use single-cell RNA sequencing to generate a comprehensive atlas of zebrafish lymphocytes during tissue homeostasis and after immune challenge. We profiled 14,080 individual cells from the gut of wild-type zebrafish, as well as of <i>rag1</i>-deficient zebrafish that lack T and B ...[more]

PMID: 30446505

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