Surface topography enhances differentiation of mesenchymal stem cells towards osteogenic and adipogenic lineages

ABSTRACT: Surface topography impacts on cell growth and differentiation, but it is not trivial to generate defined surface structures and to assess the relevance of specific topographic parameters. In this study, we have systematically compared in vitro differentiation of mesenchymal stem cells (MSCs) on a variety of groove/ridge structures. Micro- and nano-patterns were generated in polyimide using reactive ion etching or multi beam laser interference, respectively. These structures affected cell spreading and orientation of human MSCs, which was also reflected in focal adhesions morphology and size. Time-lapse demonstrated directed migration parallel to the nano-patterns. Overall, surface patterns clearly enhanced differentiation of MSCs towards specific lineages: 15 um ridges increased adipogenic

ORGANISM(S): Homo sapiens

SUBMITTER: Giulio Abagnale 

PROVIDER: E-MTAB-7177 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress