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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Gene expression profiling of Fowlpox knock-out mutant viruses using Affymetrix GeneChip Chicken Genome Arrays

ABSTRACT: Fowlpox virus (FWPV) is a double-stranded DNA virus, used as a live vaccine against poultry diseases, and considered a promising potential mammalian vaccine vector. Similar to mammalian poxviruses, FWPV has evolved mechanisms to evade host immune responses at different levels. We infected chicken embryo fibroblasts (CEF) with individual FWPV mutants (n=59), each deficient in one non-essential gene, from a previously described knock-out virus library [Laidlaw et al, 2013 J Virology 87(9); Laidlaw and Skinner, 2014 Bio-protocol 4(10); e1126]. Host responses to the mutant viruses were screened at 16h post infection for each virus using Affymetrix GeneChip Genome arrays which includes probes for most FWPV genes. Controls included the wild type virus and uninfected samples. To avoid systematic errors due to different batches of CEF and hybridisation/scanning on different dates, samples were processed in groups of randomised samples.

ORGANISM(S): Gallus gallus

SUBMITTER: Efstathios Giotis

PROVIDER: E-MTAB-7276 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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