RNA-Seq of maize inbred lines and their F1 reciprocal hybrids
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Illumina HiSeq 2500
ORGANISM(S): Zea mays
SUBMITTER: Bo Wang
PROVIDER: E-MTAB-7394 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Variant phasing and haplotypic expression from long-read sequencing in maize.
Wang Bo B Tseng Elizabeth E Baybayan Primo P Eng Kevin K Regulski Michael M Jiao Yinping Y Wang Liya L Olson Andrew A Chougule Kapeel K Buren Peter Van PV Ware Doreen D
Communications biology 20200218 1
Haplotype phasing maize genetic variants is important for genome interpretation, population genetic analysis and functional analysis of allelic activity. We performed an isoform-level phasing study using two maize inbred lines and their reciprocal crosses, based on single-molecule, full-length cDNA sequencing. To phase and analyze transcripts between hybrids and parents, we developed IsoPhase. Using this tool, we validated the majority of SNPs called against matching short-read data from embryo, ...[more]
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