Hydrogen sulfide stimulates Mycobacterium tuberculosis respiration and growth to exacerbate TB disease

ABSTRACT: The aim of this experiment was to determine how exposure of Hydrogen sulfide impacts gene expression in Mycobacterium tuberculosis. RNA was isolated from actively growing mycobacterial cells (0.6-0.8 OD600) using Trizol according to established protocols (27). Briefly, cells were exposed to 25 µM GYY4137 for 1 hr under carefully controlled conditions (n=3/group) and RNA isolated. Unexposed cells received spent GYY4137 (without any capacity to produce Hydrogen sulfide).

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Mycobacterium tuberculosis H37Rv

SUBMITTER: Vikram Saini 

PROVIDER: E-MTAB-7421 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress