Identification of candidate susceptibility and resistance genes of mice infected with Streptococcus suis type 2

ABSTRACT: There were four groups:control A/J mice;control B6 mice;SS2-infected A/J mice;SS2-infected B6 mice.The RNA samples of four groups were sent to Biostar Genechip Inc. (Shanghai, China) for microarray hybridization. The pooled RNA sample from each group was hybridized to one Illumina mouse Genome Beadchip Array (catalog number 5022612022, Mouse WG-6_V2, Illumina). Therefore, four BeadChips were used in total, one for each of the A/J and B6 infected and control mice groups. Biotin-labeled cRNA preparation and hybridization were performed as described previously. The arrays were scanned on an Illumina BeadStation 500 System and the hybridization data analyzed using Illumina BeadStudio software. The following filtering criteria were used for selection of differentially expressed genes: positive gene in either test or control, and test DiffScore e +20 or dc20. The differentially expressed genes were selected by comparing the following groups: SS2-infected A/J vs. SS2-infected B6; control A/J vs. control B6; SS2-infected A/J vs. control A/J; SS2-infected B6 vs. control B6. Four RNA samples were hybridized with four BeadChips of Illumina. They were respectively control A/J mice, control B6 mice, SS2-infected A/J mice, and SS2-infected B6 mice.The differentially expressed genes were selected by comparing the following groups: SS2-infected A/J vs. SS2-infected B6; control A/J vs. control B6; SS2-infected A/J vs. control A/J; SS2-infected B6 vs. control B6.In the raw and normalized date, A/J1 represents control A/J, A/J2 represents SS2-infected A/J, B61 represents control B6, B62 represents SS2-infected B62.

ORGANISM(S): Mus Musculus

SUBMITTER: jie rong 

PROVIDER: E-MTAB-745 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress