Project description:The transcriptional profile of pyrethroid resistant Anopheles arabiensis from Zanzibar. Anopheles arabiensis from Pemba Island, exposed (Survivors) and non-exposed (Pemba) to a discriminating dose of the pyrethroid lambda-cyhalothrin were compared to two insecticide susceptible strains from Zanzibar island (Unguja) and Dar es Salaam (Dar).
Project description:The transcriptional profile of Anopheles arabiensis collected from a pyrethroid resistant area on Pemba Island collected in 2011 was compared to that of a lab susceptible colony of Anopheles arabiensis (MOZ).
Project description:Transcriptional profiling of three Anopeheles arabiensis strains: (i) DDT resistant mosquitoes from the city of Bobo Dioulasso (ii) mosquitoes from Bobo Dioulasso not exposed to DDT (iii) a laboratory DDT-susceptible strain.
Project description:The Anopheles gambiae midgut harbors bacteria that proliferate upon a blood feed. We used microarrays to examine the midgut gene expression response at early stages (3hours) after an artifitial meal containing heat killed bacteria. Anopheles gambiae G3 mosquitoes 5-6 day-old were fed BSA (20% in PBS with fresh 10 mM sodium bicarbonate) with or without heat killed E. coli (equivalent of 2.5 ml of 0.8 OD) . Three pools of 10 mosquito midguts were dissected after 3h and processed for microarray analysis of gene expression.
Project description:Anopheles gambiae, the primary African malarial mosquito, exhibits numerous behaviors that are under diel and circadian control, including locomotor activity, swarming, mating, host seeking, eclosion, egg laying and sugar feeding. However, little has been performed to elucidate the molecular basis for these daily rhythms. To study how gene expression is globally regulated by diel and circadian mechanisms, we have undertaken a DNA microarray analysis of A. gambiae head and bodies under 12:12 light:dark cycle (LD) and constant dark (DD, free-running) conditions. Zeitgeber Time (ZT) with ZT12 defined as time of lights OFF under the light:dark cycle, and ZT0 defined as end of the dawn transition. Circadian Time (CT) with CT0 defined as subjective dawn, inferred from ZT0 of the previous light:dark cycle. Adult mated but non-blood fed female mosquito heads and bodies under 12:12 light:dark cycle (LD) and constant dark (DD) conditions were collected every 4 hr to generate 48 hr gene expression profiles, and samples were processed with Affymetrix full genome microarrays. Downstream analysis identified genes with ~24hr rhythmic expression profiles.
Project description:Transcriptome profiling of a multi insecticide resistant strain of Anopheles gambiae from Burkina Faso compared to a susceptible strain Ngousso from Cameroon.
Project description:Transcriptome profiling of three DDT resistant strains of Anopheles gambiae in Cameroon (Gare, Messa and Nkolondom- 2 M forms and one S form) compared to the susceptible strain Ngousso (M form) and Kisumu (S form). S-form is distributed across sub-Saharan Africa and breeds mostly in association with rain-dependent pools and temporary puddles. M-form distribution overlaps with that of S-form in West and Central Africa, but the former form is apparently absent east of the Great Rift Valley; it is able to exploit relatively more permanent breeding sites, often closely associated with human activities, such those created by irrigation, rice cultivation and urbanization (Santolamazza et al., 2011).
Project description:Transcriptome profiling of insecticide resistant field populations of Anopheles funestus from Benin compared to a susceptible lab strain FANG
Project description:Transcriptome profiling of pyrethroid resistant field populations of Anopheles funestus from Malawi and Mozambique compared to a susceptible lab strain FANG