Dynamic adaptation of mesenchymal stem cell physiology upon exposure to surface micropatterns

ABSTRACT: Human mesenchymal stem cells (hMSCs) are defined as multi-potent colony-forming cells expressing a specific subset of plasma membrane markers when grown on flat tissue culture polystyrene. However, as soon as hMSCs are used for transplantation, they are exposed to a 3D environment, which can strongly impact cell physiology and influence proliferation, differentiation and metabolism. Strategies to control in vivo hMSC behavior, for instance in stem cell transplantation or cancer treatment, are skewed by the un-physiological flatness of the standard well plates. We used micrometer-scale defined surface topographies as a model to describe the phenotype of hMSCs during adaptation to their new environment. Compared to hMSCs cultured on flat polystyrene, we observed dramatically changed cell mor

ORGANISM(S): Homo sapiens

SUBMITTER: Jan de Boer 

PROVIDER: E-MTAB-7505 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress