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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Bulk RNA Barcoding and sequencing (BRB-seq) protocol for RNA-seq of 60 human LCL from the 1000G project

ABSTRACT: Here, we validate a novel protocol, Bulk RNA Barcoding and sequencing (BRB-seq), that combines the multiplexing-driven cost-effectiveness of a single-cell RNA-seq protocol with the efficiency of a bulk RNA-seq procedure. For this we use BRB-seq protocol on 60 human LCL data from the 1000G project samples, in order to show its multiplexing capacity and compare its effectiveness to already published TruSeq results on same samples.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Homo sapiens

SUBMITTER: Vincent Gardeux

PROVIDER: E-MTAB-7524 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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BRB-seq: ultra-affordable high-throughput transcriptomics enabled by bulk RNA barcoding and sequencing.

Alpern Daniel D Gardeux Vincent V Russeil Julie J Mangeat Bastien B Meireles-Filho Antonio C A ACA Breysse Romane R Hacker David D Deplancke Bart B

Genome biology 20190419 1

Despite its widespread use, RNA-seq is still too laborious and expensive to replace RT-qPCR as the default gene expression analysis method. We present a novel approach, BRB-seq, which uses early multiplexing to produce 3' cDNA libraries for dozens of samples, requiring just 2 hours of hands-on time. BRB-seq has a comparable performance to the standard TruSeq approach while showing greater tolerance for lower RNA quality and being up to 25 times cheaper. We anticipate that BRB-seq will transform ...[more]

PMID: 30999927

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