Unknown,Transcriptomics,Genomics,Proteomics

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Drought treatment and ABA feeding to ABA insensitive Populus tremula × tremuloides lines

ABSTRACT: 4 weeks old rooted plantlets (P. tremula × tremuloides) of wildtype (T89) and 35S::abi1-1 lines (line 76-1 and line 76-3) were potted in soil in 1.5 l pots and were kept in a chamber with 1000 ppm CO2 supplement. The plants were grown for six weeks and well irrigated before the treatment started. Three treatments of well-watered (control), ABA feeding and drought stress were applied. Woody samples were collected after 4 weeks of treatment for RNA extraction and RNA sequencing.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Populus tremula x Populus tremuloides

SUBMITTER: Dade Yu

PROVIDER: E-MTAB-7588 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Project description:The urgent need to address water scarcity underscores the importance of enhancing plant drought resistance. This study investigates whether pretreatment with abscisic acid (ABA) activates early stress signaling, thereby improving barley drought response when subsequently exposed to drought conditions. Although the individual responses to drought and ABA are well-documented, their synergistic effects in barley warrant further investigation. This study examines the impact of ABA on barley drought resilience through an experimental design that incorporates four distinct treatments: optimal watering, ABA application at 60 days post-sowing, and two drought stress treatments - one with and the other without prior ABA application. Key physiological parameters, such as photosynthesis, stomatal conductance and chlorophyll content, were analyzed in conjunction with transcriptomics. The results suggest that ABA pretreatment initiates early stomatal closure and elevates the expression of essential genes like NCED1, BG8, and HvA22, priming barley for improved drought resistance. During the drought, ABA-pre-treated barley maintained high chlorophyll levels, indicating sustained photosynthetic activity, a trend that persisted across treatments during the post-drought recovery phase. Furthermore, ABA pre-treatment was found to preserve photosystem II efficiency during drought conditions. Transcriptomic analyses revealed distinct gene expression profiles, alternative splicing profile and isoform switching, highlighting the molecular complexities of ABA role in drought response. These alterations span stress response, metabolic pathways, and DNA modification processes, providing a comprehensive view of ABA treatment's regulatory and metabolic impacts. In conclusion, ABA pretreatment strengthens barley drought defense by fostering stomatal closure and gene activation, guiding research strategies grounded in ABA and suggesting that genotypes with elevated ABA levels could have enhanced resilience and recovery capabilities.

Dataset Information

Drought treatment and ABA feeding to ABA insensitive Populus tremula × tremuloides lines

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