Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Single cell RNA seq of influenza tetramer specific CD8+ T cells from baseline, acute and long term memory timepoints

ABSTRACT: The aim of the experiment was to assess the differentiation and activation status of influenza-specific CD8+ T cells (A2/PB1 413 and A2/BHA 543) from an individual naturally infected with influenza B (PCR-confirmed). Single tetramer+ CD8+ T cells from tetramer-enriched PBMCs were sorted from baseline (~3 months before infection), at 14 days after infections, at 3 months after infection and at 1.5 years after infection. Libraries were prepared using a modified Smart-seq2 protocol as detailed in the methods section of the manuscript.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Homo sapiens

SUBMITTER: Fabio Luciani 

PROVIDER: E-MTAB-7606 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

Human CD8<sup>+</sup> T cell cross-reactivity across influenza A, B and C viruses.

Koutsakos Marios M   Illing Patricia T PT   Nguyen Thi H O THO   Mifsud Nicole A NA   Crawford Jeremy Chase JC   Rizzetto Simone S   Eltahla Auda A AA   Clemens E Bridie EB   Sant Sneha S   Chua Brendon Y BY   Wong Chinn Yi CY   Allen E Kaitlynn EK   Teng Don D   Dash Pradyot P   Boyd David F DF   Grzelak Ludivine L   Zeng Weiguang W   Hurt Aeron C AC   Barr Ian I   Rockman Steve S   Jackson David C DC   Kotsimbos Tom C TC   Cheng Allen C AC   Richards Michael M   Westall Glen P GP   Loudovaris Thomas T   Mannering Stuart I SI   Elliott Michael M   Tangye Stuart G SG   Wakim Linda M LM   Rossjohn Jamie J   Vijaykrishna Dhanasekaran D   Luciani Fabio F   Thomas Paul G PG   Gras Stephanie S   Purcell Anthony W AW   Kedzierska Katherine K  

Nature immunology 20190218 5

Influenza A, B and C viruses (IAV, IBV and ICV, respectively) circulate globally and infect humans, with IAV and IBV causing the most severe disease. CD8<sup>+</sup> T cells confer cross-protection against IAV strains, however the responses of CD8<sup>+</sup> T cells to IBV and ICV are understudied. We investigated the breadth of CD8<sup>+</sup> T cell cross-recognition and provide evidence of CD8<sup>+</sup> T cell cross-reactivity across IAV, IBV and ICV. We identified immunodominant CD8<sup>+  ...[more]

Similar Datasets

Transcriptomics Single cell RNA-seq of human CD8+ T cells binding the IAV HLA-A24/PB1 498-505 tetramer
2021-04-08 | E-MTAB-10109 | biostudies-arrayexpress
Transcriptomics CD8+ T cell gene expression after different antigen dose stimulations
2013-12-01 | E-GEOD-39761 | biostudies-arrayexpress
Transcriptomics Expression data from CD1d tetramer-sorted b-GL122/LGL1–specific human T cells or iNKT cells
2016-02-16 | E-GEOD-77909 | biostudies-arrayexpress
Transcriptomics Total and cholera toxin B-tetramer specific T cells from Peyer's patches and mesenteric lymph nodes
2022-06-22 | E-MTAB-11805 | biostudies-arrayexpress
Unknown Antigen-specific CD8 T cells against SARS-CoV-2
2023-04-09 | GSE202262 | GEO
Transcriptomics Whole mouse genome analysis of LCMV-specific CD4 and CD8 T cells throughout acute and chronic LCMV infection.
2012-12-12 | E-GEOD-30431 | biostudies-arrayexpress
Transcriptomics Naïve-like Yellow-Fever specific CD8 T cells and reference CD8 T cell subsets in humans
2015-04-08 | E-GEOD-65804 | biostudies-arrayexpress
Transcriptomics Single-cell RNAseq of Human T lymphocyte derived from H7N9 infected patients
2018-01-30 | E-MTAB-6379 | biostudies-arrayexpress
Transcriptomics Defining memory-like CD8 T cells that respond to PD-1 therapy in chronic viral infection
2016-08-02 | E-GEOD-84105 | biostudies-arrayexpress
Unknown Single cell RNA and TCR profiling of tetramer sorted mouse mLN and IELs.
2022-09-01 | GSE196426 | GEO