Protein kinase B-mediated phosphorylation controls binding of global transcriptional regulator Lsr2 to DNA in Mycobacterium tuberculosis

ABSTRACT: Two billion people are estimated to have latent tuberculosis infection associated with dormant Mycobacterium tuberculosis. Mycobacteria possess a great portfolio of regulatory proteins which that control transitions betweento dormancy and resuscitation, including the essential protein kinase B (PknB). Here, we established that depletion of PknB resulted in global alteration of the M. tuberculosis transcriptome, and identified and implicated the DNA-binding protein Lsr2 as a main transcriptional regulator likely responsible for these changes. Furthermore, we showed that Lsr2 was phosphorylated by PknB in vitro and phosphorylation of Lsr2 on threonine 112 was important for M. tuberculosis growth and survival in the Wayne non-replicating persistence model. Our fluorescence anisotropy and ele

ORGANISM(S): Mycobacterium tuberculosis H37Rv

SUBMITTER: Simon Waddell 

PROVIDER: E-MTAB-7627 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress