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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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RNA-Seq of hippocampus in Diversity Outbred mice

ABSTRACT: To understand the genetic regulation of gene expression and patterns of gene co-expression, we sequenced the transcriptome of the hippocampus of 258 Diversity Outbred (DO) mice of both sexes. DO mice (fourth and fifth generations of outcrossing) were sacrificed between 6-8 weeks of age and hippocampus dissected. Total hippocampal RNA was isolated using a TRIzol Plus RNA purification kit (Life Technologies) and mRNA sequencing library was prepared using a TruSeq kit (Illumina), both according to manufacturer's protocols. Paired-end 100bp reads were obtained using the Illumina HiSeq 2000.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Mus musculus

SUBMITTER: Elissa Chesler

PROVIDER: E-MTAB-7637 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Reference Trait Analysis Reveals Correlations Between Gene Expression and Quantitative Traits in Disjoint Samples.

Skelly Daniel A DA Raghupathy Narayanan N Robledo Raymond F RF Graber Joel H JH Chesler Elissa J EJ

Genetics 20190521 3

Systems genetic analysis of complex traits involves the integrated analysis of genetic, genomic, and disease-related measures. However, these data are often collected separately across multiple study populations, rendering direct correlation of molecular features to complex traits impossible. Recent transcriptome-wide association studies (TWAS) have harnessed gene expression quantitative trait loci (eQTL) to associate unmeasured gene expression with a complex trait in genotyped individuals, but ...[more]

PMID: 31113812

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