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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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RNAseq of yeast cells sampled during the lag (10% glucose consumption), mid-exponential (75% glucose consumed) and post-exponential phases (>99% glucose consumed)

ABSTRACT: The aim of the study was to identify regulatory elements of promoters associated with glucose-dependent gene expression profiles. For the transcriptome analysis an overnight culture of log-phase S. cerevisiae CEN.PK-113-7D grown in Delft medium was inoculated into a 500mL Delft medium at a starting OD of 0.03We carried out biological triplicate sampling from a 1 ltr BioStat Q bioreactor at 30 degrees with 800rpm agitation, with controlled aeration and pH maintained at 6 using 2M NaOH.

INSTRUMENT(S): Illumina MiSeq, NextSeq 500

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Michael Jensen

PROVIDER: E-MTAB-7657 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Engineered Reversal of Function in Glycolytic Yeast Promoters.

Rajkumar Arun S AS Özdemir Emre E Lis Alicia V AV Schneider Konstantin K Qin Jiufu J Jensen Michael K MK Keasling Jay D JD

ACS synthetic biology 20190515 6

Promoters are key components of cell factory design, allowing precise expression of genes in a heterologous pathway. Several commonly used promoters in yeast cell factories belong to glycolytic genes, highly expressed in actively growing yeast when glucose is used as a carbon source. However, their expression can be suboptimal when alternate carbon sources are used, or if there is a need to decouple growth from production. Hence, there is a need for alternate promoters for different carbon sourc ...[more]

PMID: 31051075

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