Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

RNA-seq of 12-weeks-old male murine heart ventricles.

ABSTRACT: RNA-Seq analysis of mouse cardiac transcriptome. RNA was isolated from the ventricles of 12-weeks-old male mice. Sequencing libraries were prepared using TrueSeq Stranded RNA LT Kit Ribo-Zero Gold (Illumina, 15032619). Libraries were pair-end sequenced on a MiSeq sequencer (Illumina) and mapped to the Mus musculus reference genome GRCm38 (https://support.illumina.com/sequencing/sequencing_software/igenome.html) using TopHat2 (Kim et al., 2013). Differential gene-expression analysis between controls and the EphB4 flox mice was performed using DESeq2 (Love et al., 2014). Read counts were obtained with HTSeq (Anders et al., 2015). Genes were considered as differentially expressed when the FDR-adjusted p-value was Ôëñ0.05.

INSTRUMENT(S): do not apply, Illumina MiSeq

ORGANISM(S): Mus musculus

SUBMITTER: Noelia Díaz

PROVIDER: E-MTAB-7686 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Endothelial EphB4 maintains vascular integrity and transport function in adult heart.

Luxán Guillermo G Stewen Jonas J Díaz Noelia N Kato Katsuhiro K Maney Sathish K SK Aravamudhan Anusha A Berkenfeld Frank F Nagelmann Nina N Drexler Hannes Ca HC Zeuschner Dagmar D Faber Cornelius C Schillers Hermann H Hermann Sven S Wiseman John J Vaquerizas Juan M JM Pitulescu Mara E ME Adams Ralf H RH

eLife 20191129

The homeostasis of heart and other organs relies on the appropriate provision of nutrients and functional specialization of the local vasculature. Here, we have used mouse genetics, imaging and cell biology approaches to investigate how homeostasis in the adult heart is controlled by endothelial EphB4 and its ligand ephrin-B2, which are known regulators of vascular morphogenesis and arteriovenous differentiation during development. We show that inducible and endothelial cell-specific inactivatio ...[more]

PMID: 31782728

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Dataset Information

RNA-seq of 12-weeks-old male murine heart ventricles.

Publications

Endothelial EphB4 maintains vascular integrity and transport function in adult heart.

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