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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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RNA-Seq of Clostridium ljungdahlii with CO as the sources of carbon and energy in exponetial phase against stationary phase

ABSTRACT: More than 5 g/L ethanol was detected at 84 h in the late-exponential phase with 0.1 MPa at pH 6.0 in the batch fermentation of C. ljungdahlii grown autotrophically. Interestingly, ethanol started to be reassimilated in the stationary phase. In order to understand the metabolic flux of ethanol, comparative transcriptome between exponential and stationary phases were performed.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Clostridium ljungdahlii DSM 13528

SUBMITTER: liu ziyong

PROVIDER: E-MTAB-7753 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Ethanol Metabolism Dynamics in Clostridium ljungdahlii Grown on Carbon Monoxide.

Liu Zi-Yong ZY Jia De-Chen DC Zhang Kun-Di KD Zhu Hai-Feng HF Zhang Quan Q Jiang Wei-Hong WH Gu Yang Y Li Fu-Li FL

Applied and environmental microbiology 20200702 14

Bioethanol production from syngas using acetogenic bacteria has attracted considerable attention in recent years. However, low ethanol yield is the biggest challenge that prevents the commercialization of syngas fermentation into biofuels using microbial catalysts. The present study demonstrated that ethanol metabolism plays an important role in recycling NADH/NAD<sup>+</sup> during autotrophic growth. Deletion of bifunctional aldehyde/alcohol dehydrogenase (<i>adhE</i>) genes leads to significa ...[more]

PMID: 32414802

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Energy conservation and carbon flux arrangement in the fermentation of Clostridium ljungdahlii with CO or CO2/H2

Project description:Clostridium ljungdahlii not only utilizes CO, but also H2 as energy source during autotrophic growth. In theory, CO is a more energetically and thermodynamically favourable energy source than H2 in the gas fermentation of C. ljungdahlii. However, how C. ljungdahlii conserves energy for growth and ethanol/acetate formation grown on CO or CO2/H2 is not in great detail. In this study, C. ljungdahlii was fermented on CO and CO2/ H2 at pH 6.0 with 0.1 MPa gas pressure. C. ljungdahlii produced 27 g/L acetate, 9 g/L ethanol, 8 g/L 2,3-butanediol and traces of lactate in the presence of CO as energy source, while it produced 25.8  0.1 g/L acetate, 1.8  0.1 g/L ethanol, 0.7  0.01g/L 2,3-butanediol and trances of lactate in the same fermentation condition using H2 as energy source. Therefore, comparative transcriptomes between cells grown on CO and cells grown on H2/CO2 were performed to investigate gene expression profiles based on three biological replicates.

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Project description:Clostridium ljungdahlii not only utilizes CO, but also H2 as energy source during autotrophic growth. And C. ljungdahlii also grows in fructose fermentation. In theory, fructose is a more energetically favourable energy source than syngas in the fermentation of C. ljungdahlii. However, C. ljungdahlii grows insufficiently in fructose and produces less acetate and ethanol, compared to syngas fermentation. In this study, C. ljungdahlii wild type and mutants were fermented on fructose. C. ljungdahlii produced less ethanol than the ΔadhE1 mutant and consumed less fructose. The ΔadhE1+2 mutant cannot grow in the syngas fermentation and produced less ethanol among the three strains. The results showed that aldehyde dehydrogenase inactivation led to efficient metabolism in C. ljungdahlii and the bifunctional aldehyde/alcohol dehydrogenases inactivation led to decrease metabolism. Thus, comparative transcriptomes among cells grown on fructose of three strains were performed to investigate gene expression profiles based on three biological replicates.

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RNA-Seq of Clostridium ljungdahlii with CO as the sources of carbon and energy in exponetial phase against stationary phase

Project description:RNA-Seq of Clostridium ljungdahlii with CO as the sources of carbon and energy in exponetial phase against stationary phase

| PRJEB31729 | ENA

Gene expression of Vibrio parahaemolyticus in the early stationary phase

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