Time series RNA-seq of Naïve CD4 positive T-cells undergoing differentiation towards T-helper 1.

ABSTRACT: In eukaryotes, mRNA abundance is often a poor proxy for protein abundance. Despite this, the majority of methods used to dissect function in mammalian biology involve measurements of mRNA, with the assumption that changes observed at the mRNA level are reflected at the protein level. The inability to predict protein abundance from mRNA abundance is a major limitation when dissecting the relationship between genotype and phenotype in mammals. To understand the effect of cell-type, splice-variant selection and translation rate on the relationship between mRNA and protein abundance, we performed RNA-sequencing and mass-spectrometry proteomics of primary human naïve CD4+ T helper (NTH) cells at six time points during differentiation into T-helper type 1 (TH1) cells. For the RNA- sequencing, NT

ORGANISM(S): Homo sapiens

SUBMITTER: Olof Rundquist 

PROVIDER: E-MTAB-7775 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress