Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptional profiling of the sigma factor sigE deletion mutant of Streptomyces coelicolor (J2130) at 0, 30, 60 and 90 minutes after treatment with sub-lethal concentration of vancomycin.

ABSTRACT: SigE is a sigma factor found in Streptomyces species and is the key regulator of cell envelope stress response in Streptomyces coelicolor. To determine the regulon of SigE under conditions of cell envelope stress, 10 ug / ml vancomycin was used to induce cell envelope stress in the wild type and a sigE deletion strain of S. coelicolor. Samples were taken 0, 30, 60 and 90 minutes after addition of vancomycin and subjected to transcriptional profiling using Affymetrix arrays. Note – equivalent data for wild type Streptomyces coelicolor M600 is submitted as part of E-MEXP-3032. The data for both M600 (E-MEXP-3032) and J2130 (this submission) were generated at the same time employing the same protocols.

ORGANISM(S): Streptomyces coelicolor A3(2)

SUBMITTER: Matt Bush 

PROVIDER: E-MTAB-7814 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Defining the regulon of genes controlled by σ<sup>E</sup> , a key regulator of the cell envelope stress response in Streptomyces coelicolor.

Tran Ngat T NT   Huang Xiaoluo X   Hong Hee-Jeon HJ   Bush Matthew J MJ   Chandra Govind G   Pinto Daniela D   Bibb Maureen J MJ   Hutchings Matthew I MI   Mascher Thorsten T   Buttner Mark J MJ  

Molecular microbiology 20190429 2

The extracytoplasmic function (ECF) σ factor, σ<sup>E</sup> , is a key regulator of the cell envelope stress response in Streptomyces coelicolor. Although its role in maintaining cell wall integrity has been known for over a decade, a comprehensive analysis of the genes under its control has not been undertaken. Here, using a combination of chromatin immunoprecipitation-sequencing (ChIP-seq), microarray transcriptional profiling and bioinformatic analysis, we attempt to define the σ<sup>E</sup>  ...[more]

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