Six in situ Hi-C libraries of control and mESC auxin-inducible degrons for Scc1 and Ring1b.

ABSTRACT: In this experiment, we've examined chromatin conformation differences of E14 mESC against cohesin and Ring1b degrons. We performed a modified in situ Hi-C protocol from (Rao et al., 2014) that can be found in detail at (Díaz et al., 2018). Samples were digested with MboI restriction enzyme. The aim of the experiment was to characterize the role of cohesin and polycomb on the 3D structure of mouse chromatin.

INSTRUMENT(S): NextSeq 550

ORGANISM(S): Mus musculus

SUBMITTER: Juan Vaquerizas 

PROVIDER: E-MTAB-7816 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress