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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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RNA-seq of primary rat macrophages cultured on substrates of defined stiffness

ABSTRACT: Cells interact with their mechanical environment and respond in consequence. Mechanical cues can have a wide range of influences on cell behaviour, ranging from guidance of differentiation and cell fate to immune activation. The impact of substrate stiffness on primary macrophages - a key player in innate immunity and inflammation - had not been previously studied. We prepared bone marrow-derived macrophage cultures from adult rat hematopoietic stem cells exposed to M-CSF, and cultured these on polyacrylamide substrates of controlled stiffness (ranging from 50 to 0.1 kPa shear modulus, covering the range found in physiological tissues) for 3 days. The RNA from these cells was then extracted and sequenced.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Rattus norvegicus

SUBMITTER: Alejandro Carnicer-Lombarte

PROVIDER: E-MTAB-7900 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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