Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-Seq of murine trisomic B cell precursors expressing KRAS_G12D

ABSTRACT: To better understand the molecular bases of the oncogenic cooperation between trisomy 21 and RAS/MAPK pathway activation, KRASG12D was overexpressed in wild-type (WT) or partially trisomic (Ts1Rhr) bone marrow cells. Transduced mCherry-positive murine B cell progenitors were platted in methylcellulose to form B cell precursors colonies (CFU-preB). After a week, B220+/CD19+/CD25- B cells were harvested to perform RNA-sequencing experiments.

INSTRUMENT(S): Illumina HiSeq 4000

ORGANISM(S): Mus musculus

SUBMITTER: Thomas MERCHER

PROVIDER: E-MTAB-8005 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

RNA-Seq of murine trisomic B cell precursors expressing KRAS_G12D

Project description:RNA-Seq of murine trisomic B cell precursors expressing KRAS_G12D

| PRJEB32827 | ENA

Mandibular precursor at E13.5 in Ts65Dn Down syndrome mouse

Project description:Gene expression of mandibular precursor from embryonic day 13.5 trisomic and euploid embryos from the Ts65Dn Down syndrome mouse model. Results provide insight into importance of non-trisomic genes in organogenesis. Total RNA isolated from mandibular precursor of 13 trisomic and 11 euploid E13.5 embryos.

2010-11-02 | E-GEOD-24554 | biostudies-arrayexpress

RPE-1 and RPE-1 trisomic AHA p-c and steady state data

Project description:We developed a pulse-chase method in where fully SILAC (heavy, medium-heavy and Light) labelled cells were pulsed with Azidohomoalanine (AHA) and then chased for different length of times. In addition, steady state protein levels comparing the RPE- and RPE-1 trisomic cells by standard SILAC labeling was also acquired. These datasets contain data for the human RPE-1 and RPE-1 trisomic cell lines.

2016-10-06 | PXD004915 | Pride

RNAseq from disomic and trisomic fibroblasts and lymphoblastoids

Project description:RNA was sequenced from individuals Disomic and Trisomic for chromosome 21 to identify consistent changes in gene expression across individuals

2016-07-29 | GSE79842 | GEO

RNAseq from disomic and trisomic T cells and monocytes

Project description:RNA was sequenced from Disomic and Trisomic individuals for chromosome 21 to identify consistent changes in gene expression across individuals

2016-07-29 | GSE84531 | GEO

RNAseq from disomic and trisomic fibroblasts and lymphoblastoids

Project description:RNA was sequenced from individuals Disomic and Trisomic for chromosome 21 to identify consistent changes in gene expression across individuals Cells were cultured at subconfluency and RNA harvested for sequencing

2016-07-29 | E-GEOD-79842 | biostudies-arrayexpress

Gene expression profiling from cohesin knockdown cells obtained grown in methylcellulose

Project description:We performed RNA-sequencing in c-Kit+ cells that were infected with retroviruses expressing shRNAs for Renilla, Rad21, Smc1a, Smc3 or Stag2. These cells were grown in methylcellulose (M3434) for either one passage (P1) or replated for five passages (P5). RNA-sequencing control (Ren) and cohesin (Rad21, Smc1a, Smc3 and Stag2) knockdown cells.

2015-09-16 | E-GEOD-72368 | biostudies-arrayexpress

RNAseq from disomic and trisomic T cells and monocytes

Project description:RNA was sequenced from Disomic and Trisomic individuals for chromosome 21 to identify consistent changes in gene expression across individuals Cells were cultured at subconfluency and RNA harvested for sequencing

2016-07-29 | E-GEOD-84531 | biostudies-arrayexpress

Single Cell Analysis in trisomic tissues and organoids

Project description:Single Cell Analysis in trisomic tissues and organoids

| PRJEB49114 | ENA

shRNA cassette sequencing from disomic and trisomic fibroblasts cultured in the presence of shRNA kinome library for 14 days

Project description:Kinome-wide shRNA screen to identify kinases differentially required for survival of cells disomic and trisomic for chromosome 21

2016-07-29 | GSE79840 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data