Identification of antigens presented by MHC for vaccines against tuberculosis

ABSTRACT: The immune response against tuberculosis relies, at least in part, on CD4+ T cells. Protective vaccines require the induction of antigen-specific CD4+ T cells via mycobacterial peptides presented by MHC class-II in infected macrophages. We have purified MHC class-I and MHC-II peptides and analysed them by mass spectrometry. We have successfully identified 97 mycobacterial peptides presented by MHC-II and 54 presented by MHC-I, from 76 and 41 antigens, respectively. The sequences of selected peptides were confirmed by spectral match validation and immunogenicity evaluated by IFN-gamma ELISpot against peripheral blood mononuclear cells from volunteers vaccinated with BCG, M.tb latently infected subjects or patients with tuberculosis disease. Three antigens were expressed in viral vectors, an

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Mycobacterium tuberculosis variant bovis BCG

SUBMITTER:  

PROVIDER: E-MTAB-8057 | biostudies-arrayexpress |

SECONDARY ACCESSION(S): ERP115817

REPOSITORIES: biostudies-arrayexpress