Protein microarray-based autoantibody screening in 51 APS1 patients and 21 healthy controls

ABSTRACT: Autoimmune polyendocrine syndrome type 1 (APS1) is a monogenic disorder that features multiple autoimmune disease manifestations. It is caused by mutations in the Autoimmune regulator (AIRE) gene, which promote thymic display of thousands of peripheral tissue antigens in a process critical for establishing central immune tolerance. We here used proteome arrays to perform a comprehensive study of autoimmune targets in APS1. Probing and scanning of the protein arrays (ProtoArray® v5.0 PAH0525020, Life Technology) was conducted according to Invitrogen’s protocol for Immune Response BioMarker Profiling, using the recommended detection reagent (Alexa Fluor® 647 Goat Anti-Human IgG A21445, Invitrogen) and blocking buffer (Blocking Buffer Kit PA055, Invitrogen). The arrays were probed with sera

ORGANISM(S): Homo sapiens

SUBMITTER: Nils Landegren 

PROVIDER: E-MTAB-8097 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress