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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Microarray experiment comparing gene expression in the bar-1 mutant EW15 with wild-type strain N2 in Caenorhabditis elegans

ABSTRACT: The Wnt-pathway is a key-signalling pathway in metazoans. The beta-catenins, which include bar-1, have a function in gene expression regulation in Wnt-signalling. In this study, the effect of a loss-of-function mutation (bar-1(ga80) in the strain EW15) on gene-expression was tested. To this end age-synchronized populations of both N2 (wild-type) and EW15 were grown for 48 hours at 20 degrees Celsius, after which populations were collected and total RNA was isolated. For each genotype six replicates were collected in three batches of two replicates.

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: Mark Sterken

PROVIDER: E-MTAB-8128 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Loss-of-function of β-catenin bar-1 slows development and activates the Wnt pathway in Caenorhabditis elegans.

van der Bent M Leontien ML Sterken Mark G MG Volkers Rita J M RJ Riksen Joost A G JA Schmid Tobias T Hajnal Alex A Kammenga Jan E JE Snoek L Basten LB

Scientific reports 20140513

C. elegans is extensively used to study the Wnt-pathway and most of the core-signalling components are known. Four β-catenins are important gene expression regulators in Wnt-signalling. One of these, bar-1, is part of the canonical Wnt-pathway. Together with Wnt effector pop-1, bar-1 forms a transcription activation complex which regulates the transcription of downstream genes. The effects of bar-1 loss-of-function mutations on many phenotypes have been studied well. However, the effects on glob ...[more]

PMID: 24819947

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