Transcription profiling of mouse CH1 B-cells upon stimulation either with anti-IgM, IL-4 or with a combination of anti-IgM and IL-4

ABSTRACT: We have studied CH1 cells that undergo G1 arrest upon anti-IgM treatment after 16 hrs of stimulation. First we studied the differential gene expression under anti-IgM and IL-4 stimulation condition individually and in combination, and this revealed the affected genes to be directly or indirectly playing a role for arresting the cells in the G1 phase of the cell cycle. We then performed Western blotting experiments for the selected signaling molecule candidates from various pathways, and the phosphorylation kinetic profiles were used to study their role in regulating the gene expression under anti-IgM and/or IL-4 stimulus. Finally, we profiled how the signaling pathways are regulating the activation and deactivation of 345 transcription factors, which are responsible for regulating the anti

ORGANISM(S): Mus musculus

SUBMITTER: Henna JM-drvenpM-dM-d 

PROVIDER: E-MTAB-82 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress