Whole genome bisulfite sequencing of bovine morula derived from metabolically divergent dairy cows

ABSTRACT: Lactating cows experience transient metabolic stresses during postpartum which results in abnormal concentrations of non-esterified fatty acids and beta-hydroxybutyrate that can affect embryo-maternal communication and ultimately reproductive success. We hypothesize that metabolic challenges in lactating cows influence DNA methylation changes of the embryos prior to implantation affecting genes involved in embryo developmental competency. Therefore, we compared whole genome bisulfite sequencing (wgbs) of morulas derived from in vitro produced 2-4 cell embryos and transferred in the oviduct of metabolically profiled lactating cows until day 7. Similar stage of in vitro production (IVP) embryos were transferred to the oviduct of nulliparous heifers as a metabolically unchallenged control. Bi

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Bos taurus

SUBMITTER: Nares Trakooljul 

PROVIDER: E-MTAB-8239 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress