Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Human cortical organoids. Chronic treatment with GSK3 inhibitor CHIR99021. Bulk RNAseq.

ABSTRACT: Here we used human cortical brain organoids to probe the longitudinal impact of GSK3 inhibition through multiple developmental stages. Chronic GSK3 inhibition increased the proliferation of neural progenitors and caused massive derangement of cortical tissue architecture. Cortical organoids were differentiated as previously described (Paşca et al., 2015, doi: 10.1038/nmeth.3415.).Chronic GSK3 inhibition was performed by adding CHIR99021 (Merck SML1046) to the medium at day 0 (1 microM) and kept throughout the differentiation process until reaching the respective collection timepoints (day 18, day 50, day 100).

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Homo sapiens

SUBMITTER: Carlo Emanuele Villa 

PROVIDER: E-MTAB-8325 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

The regulation of the proliferation and polarity of neural progenitors is crucial for the development of the brain cortex. Animal studies have implicated glycogen synthase kinase 3 (GSK3) as a pivotal regulator of both proliferation and polarity, yet the functional relevance of its signaling for the unique features of human corticogenesis remains to be elucidated. We harnessed human cortical brain organoids to probe the longitudinal impact of GSK3 inhibition through multiple developmental stages  ...[more]

Similar Datasets

Transcriptomics Single cell RNAseq of human cortical organoids investigating the effect of chronic treatment with GSK3 inhibitor CHIR99021
2020-06-09 | E-MTAB-8337 | biostudies-arrayexpress
Genomics Bulk RNA-seq of human cortical organoids treated with GSK3 inhibitor CHIR99021
| PRJEB38729 | ENA
Genomics Single cell RNAseq of human cortical organoids investigating the effect of chronic treatment with GSK3 inhibitor CHIR99021
| PRJEB38791 | ENA
Genomics Gene expression profiling of GSK3 inhibitor (CHIR99021)-treated intestinal organoids
| PRJNA433318 | ENA
Unknown Polyubiquitination analysis upon GSK3 inhibition in HeLa cell extracts
2014-05-15 | GSE50629 | GEO
Unknown miRNA analysis to identify common targets for GSK3
2017-07-06 | GSE84100 | GEO
Unknown RNA sequencing to identify common targets for GSK3
2017-07-10 | GSE84302 | GEO
Transcriptomics E-MTAB-8325
2022-01-18 | E-MTAB-8325 | ExpressionAtlas
Proteomics Polyubiquitination analysis upon GSK3 inhibition in HeLa cell extracts
2014-05-15 | E-GEOD-50629 | biostudies-arrayexpress
Unknown RNA-Seq for transcriptome analysis of germinal center B cells upon GSK3 inhibition
2020-11-22 | GSE144361 | GEO