Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

6 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

MicroRNA profiling by array to investigate circulating miRNAs in pancreatic cancer compared to healthy donors

ABSTRACT: This study aimed to uncover new circulating microRNAs (miRNAs) that would distinguish patients with pancreatic cancer (PanC) from healthy subjects (HS), and predict patients’ clinical phenotypes and outcomes.

ORGANISM(S): Homo sapiens

SUBMITTER: Orazio Palumbo

PROVIDER: E-MTAB-8378 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Cell-free serum miRNA signature of synovial sarcoma

Project description:Synovial sarcomas account for approximately 10% of all soft-tissue tumors and occur most frequently in young adults. A specific translocation in this sarcoma induces fusion of the SYT gene on chromosome 18 to the SSX genes on chromosome X, leading to proliferation of the tumor cells. The need for non-invasive biomarkers indicating recurrence and activity of this disease has sparked research into short non-coding RNA known as microRNA (miRNA). Patients and Methods: Serum samples of patients with active synovial sarcoma, healthy donors and leiomyosarcoma patients were collected. Cell-free serum was obtained by differential centrifugation steps and analyzed using an Affymetrix GeneChip miRNA Array v. 4.0. qRT-PCR was carried out to confirm a panel of miRNAs which where differentially expressed in the miRNA array.

2018-09-19 | E-MTAB-3888 | biostudies-arrayexpress

Pancreatic Cancer miRNA expression profiles

Project description: Not available

2011-08-01 | E-MTAB-753 | biostudies-arrayexpress

Colon Cancer miRNA expression profiles

Project description: Not available

2011-08-01 | E-MTAB-752 | biostudies-arrayexpress

Glioma miRNA expression profile

Project description:Comparison of the miRNAs profile of 8 WHO grade II gliomas and 24 higher grade tumors (2 WHO grade III and 22 glioblastomas) in order to identify the molecular determinants of progression.

2014-03-15 | E-MTAB-1840 | biostudies-arrayexpress

Influence of RNA Labeling on Expression Profiling of MicroRNAs

Project description:Several technical parameters are now being optimized for microRNA expression profiling experiments, but the finite amount of total RNA available continues to bottleneck clinical investigations. We used microarrays to determine the influence of the labeling reagent on total RNA requirements and data quality. Human brain/lung samples were each labeled in duplicate, at 1.0ug, 0.5ug, 0.2ug, and 0.1ug of total RNA, using two microRNA labeling kits (Genisphere Biotin and Biotin-HSR) that utilize the same labeling procedure but differ in the composition of the labeling reagent used to label the microRNA molecules in the samples. A total of 32 arrays were used for this experiment. Additionally, synthetic microRNA spike-in experiments were also performed to establish the signal dynamic range of the microarray using the Biotin-HSR labeling kit. Two-fold serial dilution samples were prepared that contained from 5000 to 4.88 attomoles of miRNA. A total of 12 arrays were used for this experiment, including a negative control sample.

2012-05-21 | E-GEOD-30045 | biostudies-arrayexpress

miRNA profiling in Breast Cancer

Project description:To exploit molecular subtyping for risk stratification in breast cancer patients

2024-01-01 | E-MTAB-13550 | biostudies-arrayexpress

miRNA Expression data from maize young leaf and developing seed

Project description:miRNAs of at least 2 times expression level higher in seeds were all from the miR319, miR171 and miR166 family. In leaves, most up-regulated miRNAs included miR399a/c/d/e, miR408, miR159c/d, miR156k/j, miR160a/b/c/d/e and miR164a/b/c/d We intend to identify the up-regulated miRNAs in maize seed and screen for key miRNAs involved in maize seed development

2012-08-06 | E-GEOD-37322 | biostudies-arrayexpress

Circulating microRNA profile in aortic stenosis

Project description:We compared the circulating plasma microRNA profile in EDTA plasma in patients with aortic stenosis to a control group.

2015-08-25 | E-MTAB-2785 | biostudies-arrayexpress

In vitro microRNA screening in human pancreatic adenocarcinoma

Project description:No reliable predictors of susceptibility to gemcitabine chemotherapy exist in pancreatic ductal adenocarcinoma. MicroRNAs (miR) are epigenetic gene regulators with tumorsuppressive or oncogenic roles in various carcinomas. This study assesses chemoresistant PDAC for its specific miR expression pattern. Gemcitabine-resistant variants of PANC-1, a mutant p53 human pancreatic adenocarcinoma cell line, were established. MicroRNA screening was investigated by microarray. Gemcitabine-resistant PANC-1 (PANC-1-GR) cell clones were produced by exposing the parental cells to repeated pulsatile gemcitabine treatment over 3 days with constant sublethal concentrations followed by recovery-periods with agent-free medium until the cells recovered exponentially. Parental PANC-1 cells were treated with 0.4ÂµM gemcitabine cycles for approximately 9 months. Affymetrix GeneChip miRNA microarrays (Affymetrix UK Ltd., High Wycombe, UK) were performed in parental and chemoresistant PANC-1 cells after 17 chemotherapy cycles using the manufacturersÂ´ protocols. The samples were prepared from 1Âµg of total-RNA in accordance with the Affymetrix FlashTag Biotin HSR RNA Labeling Kit. The targets were hybridized overnight to Affymetrix GeneChip miRNA arrays. Following hybridization, the arrays were washed and stained using the Affymetrix GeneChip Fluidics Station 450 and scanned using the Affymetrix GeneChip Scanner 3000 7G. Microarray data quality was checked as recommended by the manufacturer and by the quality metrics in the Partek Genomics Suite software (Partek Inc., St. Louis, MO).

2015-11-03 | E-GEOD-74562 | biostudies-arrayexpress

Hepatic Gene Expression Changes in Diethynitrosamin-initiated and Cholic Acid promoted tumors

Project description:Mice were fed with either normal diet (ND), 0.2% cholic acid diet (0.2%CA), DEN treated and fed ND or DEN treated and fed 0.2%CA diet. DEN was treated at 15 microgram/kg body weight at postnatal day 15. Diets were fed for two months starting 8 months of age till 10 months of age. Livers were collected at10 months of age, Total RNA was isolated and used for microarray experiments. 4 groups [ND, 0.2%CA, DEN+ND and DEN+0.2%CA], pooled livers from 3-5 mice

2016-08-10 | E-GEOD-75445 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data