Complex response on the level of the transcriptome by deletion of a local transcriptional repressor AmlR by RNAseq of the coding RNA

ABSTRACT: Actinoplanes sp. SE50/110 is the wild type of industrial production strains of the fine-chemical acarbose (acarviosyl-maltose), which is used as α-glucosidase inhibitor in the treatment of type II diabetes. Although maltose is an important building block of acarbose, the maltose/maltodextrin metabolism has not been studied in Actinoplanes sp. SE50/110 yet. A PurR/LacI-type transcriptional regulator gene, named amlR (ACSP50_2475), is localized upstream of the maltase gene amlE (ACSP50_2474), which is organized in an operon with and a gene downstream (ACSP50_2473) encoding a GGDEF-EAL-domain-containing protein putatively involved in c-di-GMP signaling. A targeted gene deletion mutant of amlR was constructed by use of CRISPR/Cas9 technology. The transcription of the aml operon is significant

INSTRUMENT(S): Illumina HiSeq 1500

ORGANISM(S): Actinoplanes sp. SE50/110

SUBMITTER: Lena Schaffert 

PROVIDER: E-MTAB-8404 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress