Whole lung gene expression from BXD mouse strains

ABSTRACT: Parental and BXD mouse lines were received from Jackson Laboratory and University of Tennessee Health Science Center. All animals were housed at UTHSC before sacrifice. Mice were killed by cervical dislocation and whole lungs including blood were removed and placed in RNAlater. Total RNA was extracted from the lungs. RNA from two to five animals per strain were pooled and used for gene expression analysis. Animals used in this study were between 49 and 93 days of age. All inbred strains were profiled for both sexes, and for a given BXD strain either males or females were used. Gene expression was measured by microarrays. Several genes which showed a restricted expression to lung tissue were identified. Known QTL regions for respiratory infection susceptibility were investigated and several cis-eQTL genes were identified. Numerous cis- and trans-regulated transcripts and chromosomal intervals with strong regulatory activity were mapped.

ORGANISM(S): Mus musculus

SUBMITTER: Claudia Pommerenke 

PROVIDER: E-MTAB-848 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress