Identification of RNA-RNA interactions in Bacillus Subtilis by in vivo UV crosslinking

ABSTRACT: Small RNAs (sRNAs) are a taxonomically-restricted but transcriptomically-abundant class of post-transcriptional regulators. While potentially of importance, we know the function of few. This is in no small part because we lack global-scale methodology enabling target identification, this being especially acute in species without known RNA meeting point proteins (e.g. Hfq). We apply a combination of psoralen RNA cross-linking and Illumina-sequencing to identify RNA-RNA interacting pairs in vivo in Bacillus subtilis, resolving previously well-described interactants. Although sRNA-sRNA pairings are rare (compared with sRNA/mRNA), we identify a robust example involving the unusually conserved sRNA (RoxS/RsaE) and an unstudied sRNA that we term Regulator of small RNA A (RosA). This interaction

INSTRUMENT(S): Illumina MiSeq

ORGANISM(S): Bacillus subtilis subsp. subtilis

SUBMITTER: Emma Denham 

PROVIDER: E-MTAB-8490 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress