Single-cell RNA sequencing of the human biliary tree
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Illumina HiSeq 4000
ORGANISM(S): Homo sapiens
SUBMITTER: Daniele Muraro
PROVIDER: E-MTAB-8495 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Cholangiocyte organoids can repair bile ducts after transplantation in the human liver.
Sampaziotis Fotios F Muraro Daniele D Tysoe Olivia C OC Sawiak Stephen S Beach Timothy E TE Godfrey Edmund M EM Upponi Sara S SS Brevini Teresa T Wesley Brandon T BT Garcia-Bernardo Jose J Mahbubani Krishnaa K Canu Giovanni G Gieseck Richard R Berntsen Natalie L NL Mulcahy Victoria L VL Crick Keziah K Fear Corrina C Robinson Sharayne S Swift Lisa L Gambardella Laure L Bargehr Johannes J Ortmann Daniel D Brown Stephanie E SE Osnato Anna A Murphy Michael P MP Corbett Gareth G Gelson William T H WTH Mells George F GF Humphreys Peter P Davies Susan E SE Amin Irum I Gibbs Paul P Sinha Sanjay S Teichmann Sarah A SA Butler Andrew J AJ See Teik Choon TC Melum Espen E Watson Christopher J E CJE Saeb-Parsy Kourosh K Vallier Ludovic L
Science (New York, N.Y.) 20210201 6531
Organoid technology holds great promise for regenerative medicine but has not yet been applied to humans. We address this challenge using cholangiocyte organoids in the context of cholangiopathies, which represent a key reason for liver transplantation. Using single-cell RNA sequencing, we show that primary human cholangiocytes display transcriptional diversity that is lost in organoid culture. However, cholangiocyte organoids remain plastic and resume their in vivo signatures when transplanted ...[more]
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